Application

Detect Prosurfactant Protein B using this Anti-Prosurfactant Protein B Antibody, reacts with both C-terminus & N-terminus validated for use in ELISA, IH & WB.

Research Sub CategoryIon & Transport Channels

Research CategoryMetabolism

Immunohistochemistry: 1:4,000-1:8,000 on human tissue with and without antigen retrieval (see protocol on back) and 1:1,000-1:2,000 on adult and fetal mouse lung (see notes on back).

Immunoblot: 1:1,000-1:5,000; proprotein 42-46kDa.

ELISA: 1:500-1:1,000

Optimal dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: reacts with both C-terminus and N-terminus

Full length recombinant peptide (381 a.a.) of the human SP-B (pre)proprotein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Rabbit serum. Liquid. Contains no preservative.

Specificity

Reacts strongly with human and mouse proSP-B (detects both the N-terminal and C-terminal propeptides). Does not react with mature SP-B. On human tissue immunostains alveolar Type II cells and non-ciliated bronchiolar cells. On mouse tissue immunostains alveolar Type II cells and non-ciliated bronchiolar (Clara cells) in distal and terminal airways.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles.